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Mahmuda et al  

Full Length Research Paper

Comparative Faecal Culture Techniques for Harvesting Large Quantity of Infective (Filariform) Larvae of Strongyloides ratti (Parasitic roundworm)

 

Aliyu Mahmuda1*, Al-Zihiry Khalid Jameel Kadhim2, Zasmy Unyah3, Usman Mahmud1, Umar Salisu Ahmad4, Shehu Zaid4, Abdurrahman Hassan Jibril5.

1Department of Parasitology and Entomology, Faculty of Veterinary Medicine, Usmanu, Danfodiyo University, Sokoto, Nigeria.
2Department of Microbiology, College of Medicine, University of Thi-Qar, Iraq.
3Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Malaysia.
4Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria.
5Department of Biochemistry and Physiology, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria.

 

Corresponding author email: alibadra1213@gmail.com; Tel: +234(0)8038338028

Received November 29, 2017; Accepted December 12, 2017

 

ABSTRACT
Strongyloidiasis is still a major problem in the immunocompromised patients, as a result of hyperinfection syndrome and disseminated disease that is characterized with severe and sometimes fatal complications. Human strongyloidiasis research requires a large supply of infective (filariform) larvae of Strongyloides stercoralis. This can be achieved through in vivo maintenance of Strongyloides stercoralis in gerbils or in dogs which requires ethical concern and also impose possible risk of infection for the researcher involved. However, the rodent species of Strongyloides are related to the human species and have been used as models for human strongyloidiasis research. This study was aimed to compare and improved the methods of harvesting a large quantity of infective (filariform) larvae of Strongylodes ratti. Baermann’s Culture; the well-known and most widely used technique were compared to Modified Faecal Culture Technique (a modification of Harada Mori principle). It was observed that a total of 8.86x104of pure (non-mixed) infective larvae were harvested using the MFCT compared to 4.97x104 of mixed stages (rhabditiform larvae, filariform larvae, and free-living adult stages) of Strongyloides ratti recovered by Baermann’s technique within one week of continuous culture and harvest. The harvested infective (filariform) larvae from MFC technique were also observed to be more active with less contamination from fecal debris, bacteria and fungi in comparison to those harvested by Baermann’s technique. It was concluded that the Modified Faecal Culture Techniques (MFCT) that was employed in this study is far better than the Baermann’s method for harvesting a large number of filariform larvae of Strongyloides ratti.


Keywords: 
Strongyloidiasis, Strongyloides ratti, Modified Fecal Culture Technique (MFCT), Baermann’s culture technique.

 

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