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Kurata et al  

 

Full Length Research Paper


Glufosinate-Resistant Italian Ryegrass Populations Emerge from Glyphosate-Resistant Populations in Japan

 

Kohei Kurata1, Minoru Ichihara2, Yoshiki Ishida2, Yoshiko Shimono1, and Tohru Tominaga1*

 

1Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
2Shizuoka Prefectural Research Institute of Agriculture and Forestry, Iwata, Shizuoka 438-0803, Japan.

 

Corresponding author email: tominaga@kais.kyoto-u.ac.jp

Received March 30, 2017; Accepted April 14, 2017
ABSTRACT

 

To evaluate glufosinate resistance, we conducted a dose-response bioassay using two glyphosate-resistant Italian ryegrass (Lolium multiflorum Lam.) populations (S63 and S64) in Shizuoka, Japan. Two additional cultivars, Waseyutaka and Common, were used as the susceptible control in the experiment. At the five to six leaf stage, glufosinate was applied at a rate of 0, 0.25, 0.5, 1.0, or 2.0 kg active ingredient (a.i.) ha–1 for each of the susceptible cultivars, at a rate of 0, 0.5, 1.0, 2.0, or 4.0 kg a.i. ha–1 for S63, and at a rate of 0, 1.0, 2.0, or 4.0 kg a.i. ha–1 for S64. Almost all individuals of the two susceptible cultivars died after the application of 1.0 kg a.i. ha–1, the recommended rate for pest control, but 33 and 23% of individuals survived from the S63 and S64 populations, respectively. The ratios of the LD50 values for resistant vs. susceptible populations (R/S ratios) for S63 and S64 were 1.3 and 1.8, respectively. However, because Italian ryegrass is an obligate out crossing, wind-pollinated species, the S63 and S64 populations included both susceptible and resistant individuals and thus the R/S ratios may have been underestimated.

 

Keywords: Glufosinate, Glyphosate, Herbicide resistance, Italian ryegrass, Lolium multiflorum, Multi-resistance.

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